Chloroquine diphosphate in transfection

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    Chloroquine diphosphate in transfection


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    Chloroquine diphosphate CQ was ingeniously used to take place of phosphate salt in traditional calcium phosphate coprecipitation method for pDNA transfection. With multiple roles of CQ in the novel Ca-CQ-pDNA complex including pDNA compaction and assistance in lysosome escape, the transfection efficiency of the pDNA was significantly increased relative to the traditional method. Transfection efficiency in fibroblasts showed a linear increase 3–6-fold as the addition of chloroquine increased from 10 to 100 μM compared to plasmid alone Fig. 2 a–c. The maximum gene expression twofold increase compared to lipofectamine alone was observed in CHO cells when the cells were transfected with lipofectamine in the presence of chloroquine Fig. 2 b. Effect of chloroquine on the activity of polyoma DNA in the trans-formation of rat cells. In an initial experiment, we tested the effect of chloroquine on the polyoma transformation of rat fibro-blast cells. Rat-1 cells were transfected with polyoma DNA in the presence or absence of chloroquine diphosphate. DNA was coprecip-1297

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    Chloroquine diphosphate in transfection

    High efficiency polyoma DNA transfection of chloroquine., Enhanced plasmid DNA transfection with lysosomotropic agents in.

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  5. Conclusion Together, these results suggest that Luteolin has the capacity to induce selectively apoptotic cell death both in primary cutaneous SCC cells and in metastatic SCC cells in combination with chloroquine, an inhibitor of autophagosomal degradation. Hence, Luteolin might be a promising agent for the treatment of cutaneous SCC.

    • Autophagy Inhibitor Chloroquine Enhanced the Cell Death..
    • High efficiency polyoma DNA transfection of chloroquine treated cells..
    • The lysosomal inhibitor, chloroquine, increases cell surface..

    Chloroquine is a member of quinolone family and is a weak intercalating agent. Chloroquine is used for treating amebiasis, rheumatoid arthritis, discoid and systemic lupus erythematosus. Application DNA intercalator. Also used to increase transfection efficiency. Chloroquine diphosphate salt has been used • in in vitro antiplasmodial assays Chloroquine diphosphate salt, c6628, c18h26cin3, dna transfection, tranfection efficiency, antigen-antibody complexes dissociate, denaturing red blood cell antigens, Created Date 20021107113401Z Chloroquine is an inhibitor of the lysosomal degradation of the DNA which is taken up by the cells, so as leelee said, transfection should have been successful, albeit at a slightly lower level than if you would have added the chloroquine. In my normal transfections, I never add chloroquine and get high efficiencies nevertheless.

     
  6. theday XenForo Moderator

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  7. Elena880 Well-Known Member

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  8. Ragnar77 Well-Known Member

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